Abstract
A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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High-Throughput Screening Assays
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Listeria monocytogenes / classification*
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Listeria monocytogenes / genetics
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Listeria monocytogenes / isolation & purification*
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Listeriosis / diagnosis*
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Listeriosis / microbiology*
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Molecular Typing / methods*
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Polymorphism, Single Nucleotide*
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Virulence Factors / genetics
Substances
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Bacterial Proteins
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Virulence Factors