Biocompatibility of PLGA/sP(EO-stat-PO)-coated mesh surfaces under constant shearing stress

Eur Surg Res. 2011;47(3):118-29. doi: 10.1159/000329412. Epub 2011 Jul 20.

Abstract

Background: In order to allow inflammatory response modification and ultimately improvement in tissue remodeling, we developed a new surface modification for meshes that will serve as a carrier for other substances. Biocompatibility is tested in an animal model.

Methods: The animal model for diaphragmatic hernia repair was established in prior studies. Meshes were surface modified with star-configured PEO (polyethylene oxide)-based molecules [sP(EO-stat-PO)]. An electrospun nanoweb of short-term absorbable PLGA (polylactide-co-glycolide) with integrated sP(EO-stat-PO) molecules was applied onto the modified meshes. This coating also served as aerial sealing of the diaphragm. A final layer of hydrogel was applied to the product. Adhesive properties, defect size and mesh shrinkage were determined, and histological and immunohistochemical investigations performed after 4 months.

Results: The mean defect size decreased markedly in both modified mesh groups. Histologically and with regard to apoptosis and proliferation rate, smooth muscle cells, collagen I/III ratio and macrophage count, no statistically significant difference was seen between the 3 mesh groups.

Conclusions: In this proof-of-principle investigation, we demonstrate good biocompatibility for this surface-modified mesh compared to a standard polypropylene-based mesh. This new coating represents a promising tool as a carrier for bioactive substances in the near future.

MeSH terms

  • Animals
  • Apoptosis
  • Biocompatible Materials
  • Cell Proliferation
  • Coated Materials, Biocompatible / adverse effects
  • Coated Materials, Biocompatible / chemistry*
  • Collagen / metabolism
  • Disease Models, Animal
  • Female
  • Hernia, Diaphragmatic / metabolism
  • Hernia, Diaphragmatic / pathology
  • Hernia, Diaphragmatic / surgery
  • Herniorrhaphy / adverse effects
  • Herniorrhaphy / methods
  • Macrophages / pathology
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Polyethylene Glycols / adverse effects
  • Polyethylene Glycols / chemistry*
  • Polyglactin 910 / adverse effects
  • Polyglactin 910 / chemistry*
  • Rabbits
  • Shear Strength
  • Stress, Mechanical
  • Surgical Mesh* / adverse effects
  • Tissue Adhesions / etiology
  • Tissue Adhesions / pathology
  • Tissue Scaffolds / adverse effects
  • Tissue Scaffolds / chemistry

Substances

  • Biocompatible Materials
  • Coated Materials, Biocompatible
  • poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer
  • Polyglactin 910
  • Polyethylene Glycols
  • Collagen