Basal and induced EROD activity in the chorioallantoic membrane during chicken embryo development

A Annas; L Granberg; W Strandberg; I Brandt; E B Brittebo; B Brunström

doi:10.1016/s1382-6689(99)00030-7

Basal and induced EROD activity in the chorioallantoic membrane during chicken embryo development

Environ Toxicol Pharmacol. 1999 Dec;8(1):49-52. doi: 10.1016/s1382-6689(99)00030-7.

Authors

A Annas¹, L Granberg, W Strandberg, I Brandt, E B Brittebo, B Brunström

Affiliation

¹ Department of Pharmaceutical Biosciences, Division of Toxicology, Uppsala University, Box 594, SE-751 24 Uppsala, Sweden.

PMID: 21781941
DOI: 10.1016/s1382-6689(99)00030-7

Abstract

The chorioallantoic membrane (CAM) is a highly vascularized tissue that takes part in the respiratory exchange of gases through the eggshell. Although the CAM may be exposed to environmental contaminants, its response to pollutants has not been studied. We examined the cytochrome P4501A (CYP1A)-catalyzed deethylation of 7-ethoxyresorufin (EROD) in the CAM during chicken embryo development. EROD was constitutively present and was inducible by the aryl hydrocarbon (Ah) receptor agonist 3,3',4,4',5-pentachlorobiphenyl (PCB 126). Our results suggest the CAM as a first line of defence of the avian embryo against toxic compounds, but also as a target for CYP1A-activated chemicals.