Cytologic, immunocytologic, histopathologic and immunohistologic diagnosis of the poorly differentiated sertoli-leydig cell tumor

Acta Cytol. 2011;55(4):382-6. doi: 10.1159/000327906. Epub 2011 Jul 22.

Abstract

Background: Sertoli-Leydig cell tumors (SLCTs), also known as arrhenoblastomas, are tumors of the sex cord-stromal group of ovary and testis cancers. They comprise <1% of all ovarian tumors. They are divided into 6 categories based on the degree of differentiation and the presence of heterologous elements. However, <15% of these tumors are poorly differentiated.

Case: A 23-year-old unmarried female presented with an 8-month history of irregular menstrual cycle and abdominal pain. There were no clinical features suggesting virilization. The left salpingo-oophorectomy specimen revealed an oval ovarian mass of 11 × 7 × 4 cm in dimension. Grossly, the cut surface of the mass was yellowish white in color and solid in consistency and touch preparation was made. By applying cytology and immunocytochemistry techniques, a preliminary diagnosis suggestive of poorly differentiated SLCT was made. The tumor was confirmed as a poorly differentiated SLCT.

Conclusion: Cytology and immunocytochemistry by WT-1, melan A, vimentin and calretinin are helpful in the diagnosis of poorly differentiated SLCTs.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Biomarkers, Tumor / metabolism*
  • Calbindin 2
  • Cell Differentiation*
  • Cytodiagnosis
  • Female
  • Humans
  • Immunoenzyme Techniques
  • MART-1 Antigen / metabolism
  • Ovarian Neoplasms / diagnosis*
  • Prognosis
  • S100 Calcium Binding Protein G / metabolism
  • Sertoli-Leydig Cell Tumor / diagnosis*
  • Vimentin / metabolism
  • WT1 Proteins / metabolism
  • Young Adult

Substances

  • Biomarkers, Tumor
  • CALB2 protein, human
  • Calbindin 2
  • MART-1 Antigen
  • S100 Calcium Binding Protein G
  • Vimentin
  • WT1 Proteins