Overproduction of proteins in Escherichia coli: vectors, hosts, and strategies

Methods Enzymol. 1990:182:93-112. doi: 10.1016/0076-6879(90)82011-p.
No abstract available

MeSH terms

  • Base Sequence
  • Cloning, Molecular / methods*
  • Escherichia coli / genetics*
  • Genes
  • Genetic Vectors*
  • Molecular Sequence Data
  • Plasmids
  • Promoter Regions, Genetic
  • Protein Sorting Signals / genetics
  • Proteins / genetics
  • Proteins / isolation & purification*
  • RNA, Messenger / genetics
  • Recombinant Proteins / isolation & purification
  • Restriction Mapping
  • Transcription, Genetic

Substances

  • Protein Sorting Signals
  • Proteins
  • RNA, Messenger
  • Recombinant Proteins