Bioimaging is currently undergoing an exciting revolution. This includes all aspects of imaging from probe development, specimen preparation, and instrumentation to image analysis and quantitation. Perhaps the most exciting developments are new platforms for imaging that radically advance the capabilities for collecting high spatial and temporal resolution data. In many cases, the standard microscope has been replaced with new purpose-built platforms that are much more flexible, and enable the implementation of new imaging modalities. such as particular single molecule and superresolution imaging methods.