Lentivirus-mediated modification of pluripotent stem cells

Methods Mol Biol. 2011:767:315-31. doi: 10.1007/978-1-61779-201-4_23.

Abstract

Relatively safe, HIV-1-based lentiviral vectors have served as an efficient means of transducing human embryonic stem cells (hESCs). Here we describe the variety of lentiviral vector systems available with the basic strategy for designing viral vectors and methods for generating viruses for efficiently infecting and selecting transduced hESCs.

MeSH terms

  • Cells, Cultured
  • Clone Cells
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism
  • Embryonic Stem Cells / virology
  • Green Fluorescent Proteins / metabolism
  • HEK293 Cells
  • Humans
  • Lentivirus / drug effects
  • Lentivirus / metabolism*
  • Lentivirus / physiology
  • Neomycin / pharmacology
  • Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / drug effects
  • Pluripotent Stem Cells / metabolism*
  • Pluripotent Stem Cells / virology*
  • Promoter Regions, Genetic / genetics
  • Transduction, Genetic / methods*
  • Transfection
  • Transgenes / genetics
  • Virus Assembly / drug effects

Substances

  • Green Fluorescent Proteins
  • Neomycin