Evaluation of pooling strategies for acute HIV-1 infection screening using nucleic acid amplification testing

J Clin Microbiol. 2011 Oct;49(10):3667-8. doi: 10.1128/JCM.00650-11. Epub 2011 Aug 10.

Abstract

A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a specimen with 1,827 copies/ml was nonreactive in one of three replicates.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Mass Screening / methods*
  • Molecular Diagnostic Techniques / methods*
  • Plasma / virology*
  • RNA, Viral / blood
  • RNA, Viral / genetics
  • Specimen Handling / methods*
  • Virology / methods*

Substances

  • RNA, Viral