Cell-like systems with riboswitch controlled gene expression

Chem Commun (Camb). 2011 Oct 14;47(38):10734-6. doi: 10.1039/c1cc13930d. Epub 2011 Aug 25.

Abstract

Synthetic riboswitches can be used to control protein expression under fully defined conditions in vitro, in water-in-oil emulsions, and in vesicles. The developed system could serve as a foundation for the construction of cellular mimics that respond to molecules of our choosing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Escherichia coli / metabolism
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Microscopy, Fluorescence
  • RNA, Messenger / metabolism
  • Ribosomes / metabolism
  • Riboswitch*
  • Theophylline / chemistry
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Luminescent Proteins
  • RNA, Messenger
  • Riboswitch
  • yellow fluorescent protein, Bacteria
  • Theophylline