Assessing G1-to-S-phase progression after genotoxic stress

Methods Mol Biol. 2011:782:221-30. doi: 10.1007/978-1-61779-273-1_16.

Abstract

Maintenance of genomic integrity is critical for the survival of organisms. Thus, mammalian cells employ a complex DNA damage response that can sense and repair DNA damage. One important aspect of the cellular DNA damage response is the activation of checkpoints that result in cell cycle arrest. In this chapter we present methods for the induction of genotoxic stress. Additionally, we describe methods for studying the progression of cells from G(1) to S phase after genotoxic stress.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bleomycin / pharmacology
  • Bromodeoxyuridine / pharmacology
  • Cell Cycle / drug effects
  • Cell Cycle / genetics
  • Cell Line, Tumor
  • DNA Damage / drug effects*
  • DNA Damage / genetics
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • G1 Phase / drug effects*
  • G1 Phase / genetics
  • Humans
  • Propidium / pharmacology
  • S Phase / drug effects*
  • S Phase / genetics

Substances

  • Bleomycin
  • Propidium
  • Bromodeoxyuridine