Purpose: To determine the effect of bone morphogenetic protein-2(BMP-2) on osteogenesis of bone mesenchymal stem cells(BMSCs) in rats.
Methods: The primary culture of rat BMSCs was succeeded and then was identified. The rat BMSCs were infected with the recombinant lentivirus with BMP-2(Lenti-BMP-2). The osteogenic effect of BMP-2 was observed. In addition, adhesive ability of BMP-2-BMSCs was detected through adhesion assay and expression of osteogenic factors OPN,OCN,Col-I and smad was observed by RT-PCR and Western blot. SPSS11.0 software package was used for analysis.
Results: Rat BMSCs were cultured and identified successfully. The osteogenic effect of BMSCs was improved by BMP-2. Lenti-BMP-2 BMSCs adhesive potential enhanced and osteogenic factors were up-regulated.
Conclusions: BMP-2 may facilitate the osteogenic effect of the rat BMSCs and would provide favourable cell resource for tissue engineering.