Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori

Insect Biochem Mol Biol. 2011 Nov;41(11):902-8. doi: 10.1016/j.ibmb.2011.08.004. Epub 2011 Sep 10.

Abstract

Epigenetic information is encoded in post-translational modifications (PTMs) of histones. Various combinations of these marks contribute to the regulation of chromatin-templated DNA metabolisms. The histone code is gradually translated into biological responses in model organisms. However, in the silkworm, the modifications of histones with unique holocentric chromosomes have not yet been analyzed. TAU-PAGE analysis of the silkworm histone variants H2A, H2B, and H3, separated by RP-HPLC, suggested silkworm specific modification. Detailed mass spectrometry analyses of the peptides derived from the N-terminus of the silkworm H3.2 generated by glutamyl endopeptidase, lysyl endopeptidase, and trypsin digestions revealed global modifications around H3K9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombyx / chemistry
  • Bombyx / genetics
  • Bombyx / metabolism*
  • Cell Line
  • Chromatography, High Pressure Liquid
  • Histones / chemistry
  • Histones / isolation & purification
  • Histones / metabolism*
  • Mass Spectrometry
  • Protein Processing, Post-Translational*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Histones