A proteome reference map of the causative agent of melioidosis Burkholderia pseudomallei

J Biomed Biotechnol. 2011:2011:530926. doi: 10.1155/2011/530926. Epub 2011 Sep 22.

Abstract

Burkholderia pseudomallei is the etiologic agent of melioidosis. Using 2DE and MALDI-TOF MS, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. We identified 282 protein spots representing 220 ORFs; many of them have been implicated in bacterial pathogenesis. Up to 20% of identified ORFs belong to post-translational modification and stress responses. The proteome reference map will support future analysis of the bacterial gene and environmental regulation and facilitate comparative proteomics with its sibling species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Burkholderia pseudomallei / genetics
  • Burkholderia pseudomallei / metabolism*
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Humans
  • Melioidosis / microbiology*
  • Protein Processing, Post-Translational / genetics
  • Proteome / genetics
  • Proteome / metabolism*
  • Reference Standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Stress, Physiological / genetics

Substances

  • Bacterial Proteins
  • Proteome