Abstract
The hepatitis B virus X gene product trans activates transcription from a variety of viral and cellular regulatory elements. We expressed the complete, nonfused X protein in Escherichia coli and showed it to be active in trans activating a human immunodeficiency virus long terminal repeat-linked chloramphenicol acetyltransferase reporter gene.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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Cloning, Molecular
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Escherichia coli / genetics*
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Gene Expression
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HIV / genetics
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Hepatitis B virus / genetics*
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Plasmids
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Recombinant Fusion Proteins / metabolism
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Repetitive Sequences, Nucleic Acid
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Restriction Mapping
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Trans-Activators / genetics
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Trans-Activators / isolation & purification
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Trans-Activators / metabolism*
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Transcriptional Activation
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Viral Regulatory and Accessory Proteins
Substances
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Recombinant Fusion Proteins
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Recombinant Proteins
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Trans-Activators
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Viral Regulatory and Accessory Proteins
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hepatitis B virus X protein
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Chloramphenicol O-Acetyltransferase