The pigment stoichiometry in a chlorophyll a/c type photosynthetic antenna

Ritsuko Fujii; Mamiko Kita; Matsumi Doe; Yoshiro Iinuma; Naohiro Oka; Yuki Takaesu; Tomonori Taira; Masahiko Iha; Tadashi Mizoguchi; Richard J Cogdell; Hideki Hashimoto

doi:10.1007/s11120-011-9698-1

The pigment stoichiometry in a chlorophyll a/c type photosynthetic antenna

Photosynth Res. 2012 Mar;111(1-2):165-72. doi: 10.1007/s11120-011-9698-1. Epub 2011 Oct 14.

Authors

Ritsuko Fujii¹, Mamiko Kita, Matsumi Doe, Yoshiro Iinuma, Naohiro Oka, Yuki Takaesu, Tomonori Taira, Masahiko Iha, Tadashi Mizoguchi, Richard J Cogdell, Hideki Hashimoto

Affiliation

¹ The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka, 558-8585, Japan. ritsuko@sci.osaka-cu.ac.jp

PMID: 21997123
DOI: 10.1007/s11120-011-9698-1

Abstract

The trimeric fucoxanthin-chlorophyll a/c protein (FCP) was purified from a Japanese brown alga, Cladosiphon okamuranus TOKIDA. Its pigment stoichiometry was determined to be chlorophyll (Chl) a:Chl c (1):Chl c (2):fucoxanthin = 4.6:1.1:1.0:5.5 by a combination of binary HPLC and (1)H NMR spectroscopy. No violaxanthin found bound to the FCP. The ratio of Chl c/Chl a in this FCP is amongst the highest so far reported.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorophyll Binding Proteins / chemistry*
Chlorophyll Binding Proteins / isolation & purification
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Magnetic Resonance Spectroscopy
Phaeophyceae / chemistry*
Photosynthesis

Substances

Chlorophyll Binding Proteins