Abstract
Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.
MeSH terms
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Bacteria / classification*
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Bacteria / genetics
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Bacteria / isolation & purification*
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Biodiversity*
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Bronchopneumonia / microbiology*
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Chronic Disease
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Cluster Analysis
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Cystic Fibrosis / complications*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Humans
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Molecular Sequence Data
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/JN802672
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GENBANK/JN802673
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GENBANK/JN802674
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GENBANK/JN802675
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GENBANK/JN802676
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GENBANK/JN802677
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GENBANK/JN802678
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GENBANK/JN802679
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GENBANK/JN802680
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GENBANK/JN802681
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GENBANK/JN802682
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GENBANK/JN802683
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GENBANK/JN802684
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GENBANK/JN802685
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GENBANK/JN802686
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GENBANK/JN802687
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GENBANK/JN802688
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GENBANK/JN802689
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GENBANK/JN802690
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GENBANK/JN802691
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GENBANK/JN802692
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GENBANK/JN802693
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GENBANK/JN802694
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GENBANK/JN802695
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GENBANK/JN802696
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GENBANK/JN802697
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GENBANK/JN802698
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GENBANK/JN802699
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GENBANK/JN802700
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GENBANK/JN802701
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GENBANK/JN802702
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GENBANK/JN802703
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GENBANK/JN802704