Sensitive and specific modified Hodge test for KPC and metallo-beta- lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain, Klebsiella pneumoniae ATCC 700603

J Clin Microbiol. 2011 Dec;49(12):4301-3. doi: 10.1128/JCM.05602-11. Epub 2011 Oct 19.

Abstract

We evaluated the ability of the modified Hodge test to discriminate between KPC- and metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolates and carbapenemase nonproducers. With Escherichia coli ATCC 25922 as the indicator strain, the MHT resulted in low sensitivity, specificity, and repeatability. Replacing the indicator strain with Klebsiella pneumoniae ATCC 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeatability, respectively).

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacteriological Techniques / methods*
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Humans
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / growth & development*
  • Pseudomonas aeruginosa / enzymology*
  • Sensitivity and Specificity
  • beta-Lactamases / metabolism*
  • beta-Lactams / pharmacology*

Substances

  • Anti-Bacterial Agents
  • beta-Lactams
  • beta-Lactamases