[Characterization of hepatitis C virus in chronic hepatitis patients: genotypes in the State of Amazonas, Brazil]

Rev Soc Bras Med Trop. 2011 Oct;44(5):638-40. doi: 10.1590/s0037-86822011000500024.
[Article in Portuguese]

Abstract

Introduction: In the State of Amazonas, data regarding the prevalence of different genotypes of hepatitis C virus remains scarce.

Methods: The genotype of 69 HCV positive patients was determined. An in-house standardized nested-PCR was used to detect HCV RNA. Genotype assignment was based on type-specific motifs on the sequenced amplicons delimited by primers HC11/HC18 from the 5' untranslated region.

Results: Of the 69 patients studied, 65.2% were male and 34.8% were female. Genotype 1 showed the greatest prevalence, followed by 3 and 2.

Conclusions: These data suggesting that Manaus is the point of arrival of HCV in the State of Amazonas.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • Female
  • Genotype
  • Hepacivirus / genetics*
  • Hepatitis C, Chronic / virology*
  • Humans
  • Male
  • Middle Aged
  • RNA, Viral / analysis*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • RNA, Viral