Effects of temperature generated from the Holmium: YAG laser on human osteoblasts in monolayer tissue culture

Methods Mol Biol. 2012:806:377-83. doi: 10.1007/978-1-61779-367-7_24.

Abstract

With the use of lasers for ablation purposes in spinal surgery, the tissue temperature increases above the boiling point of water, leading to tissue ablation by vaporisation. Due to the thermal environment engendered by the use of lasers, there is concern about the safety of the surrounding important structures, such as dura mater, dorsal root ganglia, and nerve roots.

MeSH terms

  • Cell Separation / methods
  • HSP70 Heat-Shock Proteins / metabolism
  • Heat-Shock Response
  • Holmium*
  • Hot Temperature*
  • Humans
  • Lasers, Solid-State*
  • Osteoblasts / cytology
  • Osteoblasts / metabolism*
  • Osteoblasts / radiation effects*
  • Primary Cell Culture / methods*

Substances

  • HSP70 Heat-Shock Proteins
  • Holmium