The innervation of the phrenic motor nucleus in the cat by serotonin-containing neurons has been studied using retrograde tracing combined with immunohistochemistry at the electron microscope level. It was found that phrenic motoneuron cell bodies and dendrites are contacted by serotonin-immunoreactive synaptic terminals. This finding suggests that the activity of phrenic motoneurons is directly affected by serotonergic neurons.