A novel real-time PCR method for determination and quantification of each cytomegalovirus glycoprotein H subtype in clinical samples

J Clin Microbiol. 2012 Feb;50(2):499-501. doi: 10.1128/JCM.06032-11. Epub 2011 Nov 23.

Abstract

To investigate reinfection in patients with congenital cytomegalovirus (CMV) infection, we established a CMV subtype-specific real-time quantitative PCR method targeting the CMV gH epitope region that can be used for evaluating pathogenic CMV strains in cases of mixed CMV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coinfection / diagnosis
  • Coinfection / virology
  • Cytomegalovirus / classification*
  • Cytomegalovirus / genetics
  • Cytomegalovirus / isolation & purification
  • Cytomegalovirus Infections / diagnosis*
  • Cytomegalovirus Infections / virology*
  • Humans
  • Infant, Newborn
  • Molecular Diagnostic Techniques / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Recurrence
  • Viral Envelope Proteins / genetics*
  • Virology / methods*

Substances

  • Viral Envelope Proteins
  • glycoprotein H, Cytomegalovirus