The polydispersity of αB-crystallin is rationalized by an interconverting polyhedral architecture

Andrew J Baldwin; Hadi Lioe; Gillian R Hilton; Lindsay A Baker; John L Rubinstein; Lewis E Kay; Justin L P Benesch

doi:10.1016/j.str.2011.09.015

The polydispersity of αB-crystallin is rationalized by an interconverting polyhedral architecture

Structure. 2011 Dec 7;19(12):1855-63. doi: 10.1016/j.str.2011.09.015.

Authors

Andrew J Baldwin¹, Hadi Lioe, Gillian R Hilton, Lindsay A Baker, John L Rubinstein, Lewis E Kay, Justin L P Benesch

Affiliation

¹ Department of Molecular Genetics, University of Toronto, 1 Kings College Circle, Toronto, ON M5S 1A8, Canada. ajb204@pound.med.utoronto.ca

PMID: 22153508
DOI: 10.1016/j.str.2011.09.015

Abstract

We report structural models for the most abundant oligomers populated by the polydisperse molecular chaperone αB-crystallin. Subunit connectivity is determined by using restraints obtained from nuclear magnetic resonance spectroscopy and mass spectrometry measurements, enabling the construction of various oligomeric models. These candidate structures are filtered according to their correspondence with ion-mobility spectrometry data and cross-validated by using electron microscopy. The ensuing best-fit structures reveal the polyhedral architecture of αB-crystallin oligomers, and provide a rationale for their polydispersity and facile interconversion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Resonance Spectroscopy
Mass Spectrometry
Models, Molecular
Protein Structure, Quaternary
alpha-Crystallin B Chain / chemistry*

Substances

alpha-Crystallin B Chain

Grants and funding

Canadian Institutes of Health Research/Canada