Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies

My D Sam; Robert T Clubb

doi:10.1007/978-1-61779-480-3_13

Preparation and optimization of protein-DNA complexes suitable for detailed NMR studies

Methods Mol Biol. 2012:831:219-32. doi: 10.1007/978-1-61779-480-3_13.

Authors

My D Sam¹, Robert T Clubb

Affiliation

¹ Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

Abstract

This chapter describes the methods to form and optimize samples of protein-DNA complexes that are suitable for detailed structure and dynamics studies by NMR spectroscopy.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

DNA / chemistry*
DNA / metabolism
Macromolecular Substances / chemistry*
Macromolecular Substances / metabolism
Nuclear Magnetic Resonance, Biomolecular / methods*
Protein Binding
Proteins / chemistry*
Proteins / metabolism

Substances

Macromolecular Substances
Proteins
DNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding