Multiple strains of Mycobacterium bovis revealed by molecular typing in a herd of cattle

Vet J. 2012 Jul;193(1):296-8. doi: 10.1016/j.tvjl.2011.11.006. Epub 2011 Dec 16.

Abstract

Mycobacterium bovis isolates from an outbreak of bovine tuberculosis in a herd of cattle in Rio de Janeiro, Brazil, were analysed by spoligotyping and variable-number tandem repeat PCR analysis of the mycobacterial interspersed repetitive unit and exact tandem repeats. Molecular typing revealed a high genetic diversity of strains in the herd. The genetic diversity could be explained by the introduction of infected animals from different sources.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / veterinary
  • Brazil / epidemiology
  • Cattle
  • Colony Count, Microbial / veterinary
  • DNA, Bacterial / genetics*
  • Dairying
  • Female
  • Genetic Variation
  • Genotype
  • Minisatellite Repeats*
  • Mycobacterium bovis / classification*
  • Mycobacterium bovis / genetics
  • Mycobacterium bovis / isolation & purification*
  • Polymerase Chain Reaction / veterinary
  • Tuberculosis, Bovine / epidemiology
  • Tuberculosis, Bovine / microbiology*

Substances

  • DNA, Bacterial