The transcriptome of the adenovirus infected cell

Virology. 2012 Mar 15;424(2):115-28. doi: 10.1016/j.virol.2011.12.006. Epub 2012 Jan 10.

Abstract

Alternations of cellular gene expression following an adenovirus type 2 infection of human primary cells were studied by using superior sensitive cDNA sequencing. In total, 3791 cellular genes were identified as differentially expressed more than 2-fold. Genes involved in DNA replication, RNA transcription and cell cycle regulation were very abundant among the up-regulated genes. On the other hand, genes involved in various signaling pathways including TGF-β, Rho, G-protein, Map kinase, STAT and NF-κB stood out among the down-regulated genes. Binding sites for E2F, ATF/CREB and AP2 were prevalent in the up-regulated genes, whereas binding sites for SRF and NF-κB were dominant among the down-regulated genes. It is evident that the adenovirus has gained a control of the host cell cycle, growth, immune response and apoptosis at 24 h after infection. However, efforts from host cell to block the cell cycle progression and activate an antiviral response were also observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Infections, Human / genetics*
  • Adenovirus Infections, Human / metabolism
  • Adenovirus Infections, Human / virology
  • Adenoviruses, Human / physiology*
  • Cells, Cultured
  • Fibroblasts / metabolism
  • Fibroblasts / virology
  • Gene Expression Profiling
  • Humans
  • Lung / cytology
  • Lung / metabolism
  • Lung / virology
  • Signal Transduction
  • Transcriptome*