Synthesis and antihormonal properties of novel 11β-benzoxazole-substituted steroids

Bioorg Med Chem Lett. 2012 Feb 15;22(4):1705-8. doi: 10.1016/j.bmcl.2011.12.110. Epub 2011 Dec 30.

Abstract

Early studies led to the identification of 11β-aryl-4',5'-dihydrospiro[estra-4,9-diene-17β,4'-oxazole] analogs with potent and more selective antiprogestational activity compared to antiglucocorticoid activity than mifepristone. In the present study, we replaced the 4'-dimethylaminophenyl group of mifepristone with the benzoxazol group to give 5a-d. We also prepared the 17β-formamido analogs 6a,b using a new synthetic strategy via the intermediate epoxide 21. These compounds were evaluated for their antagonist hormonal properties using the T47D cell-based alkaline phosphatase assay and the A549 cell-based functional assay. Compound 5c showed potent antagonist activity at GR with better selectivity for GR versus PR than mifepristone and is a promising lead for further development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzoxazoles / chemical synthesis
  • Benzoxazoles / chemistry
  • Benzoxazoles / pharmacology
  • Cell Line, Tumor
  • Hormone Antagonists / chemical synthesis*
  • Hormone Antagonists / chemistry
  • Hormone Antagonists / pharmacology*
  • Humans
  • Inhibitory Concentration 50
  • Mifepristone / chemistry
  • Molecular Structure
  • Receptors, Glucocorticoid / antagonists & inhibitors*
  • Receptors, Progesterone / antagonists & inhibitors*
  • Steroids / chemical synthesis*
  • Steroids / chemistry
  • Steroids / pharmacology*
  • Substrate Specificity / drug effects

Substances

  • Benzoxazoles
  • Hormone Antagonists
  • Receptors, Glucocorticoid
  • Receptors, Progesterone
  • Steroids
  • Mifepristone