The simian immunodeficiency virus (SIV) rev gene regulates env expression

J Med Primatol. 1990;19(3-4):167-76.

Abstract

The rev gene product is a trans-acting nuclear regulatory protein that is essential for AIDS virus replication. To define the effect of the SIV rev gene on its gp160 expression, two SV40 constructs have been made: pBC17 is a rev+env+ construct, and pBD21 is a rev-env+ construct. After transfecting the constructs into COS-1 cells, RNA, protein, syncytium formation, and monoclonal antibody blocking assays were performed. The results indicated that the SIV rev gene positively influenced the level of full-length env mRNA and was required for expression of SIV gp160.

MeSH terms

  • Antibodies, Monoclonal
  • Blotting, Northern
  • Cell Line
  • Cloning, Molecular
  • Gene Expression Regulation, Viral*
  • Gene Products, env / genetics*
  • Gene Products, env / metabolism
  • Genes, env*
  • Genes, rev*
  • Giant Cells / metabolism
  • HIV Envelope Protein gp160
  • Humans
  • Plasmids
  • Precipitin Tests
  • Protein Precursors / genetics*
  • Protein Precursors / metabolism
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Viral / analysis
  • RNA, Viral / genetics
  • Simian Immunodeficiency Virus / genetics*
  • Transcriptional Activation
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Gene Products, env
  • HIV Envelope Protein gp160
  • Protein Precursors
  • RNA, Messenger
  • RNA, Viral