Abstract
We report that type I interferons (IFNs) upregulate latent membrane protein 1 (LMP-1) expression by direct activation of the ED-L1 promoter in several Epstein-Barr virus (EBV)-carrying Burkitt's lymphoma lines. In EBV-infected primary B cells, IFN-α transiently upregulates LMP-1 mRNA, but not protein levels, followed by downregulation of both, suggesting a novel antiproliferative mechanism of type I IFNs. Furthermore, our results may explain the expression of LMP-1 in memory B cells of systemic lupus erythematosus patients.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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B-Lymphocytes / drug effects
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B-Lymphocytes / metabolism*
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B-Lymphocytes / virology
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Cell Line
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Gene Expression Regulation, Viral / drug effects
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Herpesvirus 4, Human / genetics*
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Herpesvirus 4, Human / immunology
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Humans
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Interferon Type I / metabolism*
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Interferon Type I / pharmacology
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Molecular Sequence Data
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Promoter Regions, Genetic
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Signal Transduction / drug effects
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Transcription, Genetic
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Viral Matrix Proteins / genetics*
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Viral Matrix Proteins / metabolism
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Virus Latency
Substances
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EBV-associated membrane antigen, Epstein-Barr virus
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Interferon Type I
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Viral Matrix Proteins
Associated data
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GENBANK/HE653895
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GENBANK/HE653896