Abstract
A pair of isotope-coded, fluorous photoaffinity labeling reagents has been developed and coupled with a peptide. The modified peptides form adducts with methanol upon light illumination, which show characteristic isotope labeling patterns in mass spectra and can be separated from other peptides through fluorous silica.
MeSH terms
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Chromatography, Liquid
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Deuterium
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Isotope Labeling
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Light
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Mass Spectrometry
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Methanol / chemistry
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Peptides / analysis*
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Peptides / chemistry
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Photoaffinity Labels / chemical synthesis*
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Protein Interaction Mapping / methods*
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Proteomics
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Silicon Dioxide / chemistry
Substances
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Peptides
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Photoaffinity Labels
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Silicon Dioxide
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Deuterium
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Methanol