Abstract
A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples.
Publication types
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Comparative Study
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Evaluation Study
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Multicenter Study
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Research Support, N.I.H., Extramural
MeSH terms
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Adolescent
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Adult
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Aged
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Aged, 80 and over
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Antigens, Protozoan / analysis*
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Child
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Child, Preschool
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Clinical Laboratory Techniques / methods*
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Cryptosporidium / isolation & purification*
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Entamoeba histolytica / isolation & purification*
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Enzyme-Linked Immunosorbent Assay / methods
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Feces / parasitology*
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Female
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Giardia / isolation & purification*
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Humans
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Infant
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Male
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Middle Aged
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Parasitology / methods*
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Sensitivity and Specificity
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Young Adult