Mutational analysis of enhancer domains responsive to trans-activation by the X gene of human hepatitis B virus

G Goodarzi; H Ohno; R Adams; A Darabi; A Tewari; M Watabe; K Watabe

doi:10.1007/BF01310752

Mutational analysis of enhancer domains responsive to trans-activation by the X gene of human hepatitis B virus

Arch Virol. 1990;114(3-4):237-42. doi: 10.1007/BF01310752.

Authors

G Goodarzi¹, H Ohno, R Adams, A Darabi, A Tewari, M Watabe, K Watabe

Affiliation

¹ Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield.

PMID: 2241574
DOI: 10.1007/BF01310752

Abstract

The X gene product of human hepatitis B virus (HBV) trans-activates the HBV enhancer. In order to identify domains responsive to trans-activation by the X gene, we introduced a series of mutations into the HBV enhancer and assayed the enhancer activities in the presence of the X gene product. Our results suggest that the EP domain of the enhancer is essential for trans-activation by the X gene.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Chloramphenicol O-Acetyltransferase / genetics
Cloning, Molecular
DNA, Viral
Enhancer Elements, Genetic*
Escherichia coli / genetics
Gene Expression Regulation, Viral*
Genes, Viral*
Hepatitis B virus / genetics*
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Open Reading Frames
Trans-Activators / physiology*
Tumor Cells, Cultured
Viral Regulatory and Accessory Proteins

Substances

DNA, Viral
Trans-Activators
Viral Regulatory and Accessory Proteins
hepatitis B virus X protein
Chloramphenicol O-Acetyltransferase