Effects of extracorporeal shock-wave lithotripsy directed at the parotid gland on oxidative stress parameters and some trace element levels in facial nerve of rats

Muscle Nerve. 2012 Apr;45(4):562-6. doi: 10.1002/mus.22326.

Abstract

Introduction: This study was designed to assess the effect of extracorporeal shock-wave lithotripsy (ESWL) exposure of the parotid gland on oxidative stress and some trace element levels in the facial nerves of rats.

Methods: Twelve male Wistar albino rats were divided into two groups, each consisting of 6 animals. The rats in the first group served as controls. The left parotid glands of animals in the second group were treated with 1000 18-kV shock waves while anesthetized with ketamine. The animals in both groups were euthanized 72 h after the ESWL treatment, and the right facial nerve was harvested for determination of oxidant/antioxidant status and trace element levels.

Results: Lipid peroxidation product malondialdehyde (MDA) and antioxidant glutathione (GSH) levels increased, and the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), decreased in the facial nerves of ESWL-treated rats. The levels of iron, lead, manganese, and cobalt increased, and magnesium, cadmium, and copper levels decreased.

Conclusions: ESWL treatment of the parotid gland may increase lipid peroxidation and decrease antioxidant enzyme activity in adjacent tissues such as the facial nerve. It also causes a decrease or increase in many mineral levels of the facial nerve, which is an undesirable condition for normal physiological function.

MeSH terms

  • Animals
  • Catalase / metabolism
  • Facial Nerve / metabolism*
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation / physiology
  • Lithotripsy / adverse effects*
  • Male
  • Malondialdehyde / metabolism
  • Metals, Heavy / metabolism
  • Oxidative Stress / physiology*
  • Parotid Gland / enzymology
  • Parotid Gland / physiology*
  • Rats
  • Rats, Wistar
  • Salivary Gland Calculi / therapy
  • Superoxide Dismutase / metabolism
  • Trace Elements / metabolism*

Substances

  • Metals, Heavy
  • Trace Elements
  • Malondialdehyde
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase