[Experimental studies on anticoagulant activity of different extract of leech in vitro]

Zhong Yao Cai. 2004 Feb;27(2):123-5.
[Article in Chinese]

Abstract

Objective: To study the anticoagulant activity of different extracts of Leech.

Methods: Prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT) were determined by coagulometer for blood samples collected from abdominal vein of Wistar rat.

Results: PT, TT, APTT were prolonged remarkably by ethyl acetate part of Leech. PT was prolonged remarkably by hexane part and aqueous solution part of Leech. APTT was prolonged remarkably by n-Butylalcohol part of Leech.

Conclusion: The different extracts of Leech have obvious anticoagulant effects. There are new anticoagulant components different from hirudin in Leech.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates
  • Animals
  • Anticoagulants / isolation & purification
  • Anticoagulants / pharmacology*
  • Blood Coagulation / drug effects*
  • Blood Coagulation Tests
  • Leeches* / chemistry
  • Male
  • Materia Medica / isolation & purification
  • Materia Medica / pharmacology*
  • Partial Thromboplastin Time
  • Prothrombin Time
  • Rats
  • Rats, Wistar
  • Solvents / chemistry
  • Thrombin Time

Substances

  • Acetates
  • Anticoagulants
  • Materia Medica
  • Solvents
  • ethyl acetate