A reverse genetics approach to study feline infectious peritonitis

J Virol. 2012 Jun;86(12):6994-8. doi: 10.1128/JVI.00023-12. Epub 2012 Apr 4.

Abstract

Feline infectious peritonitis (FIP) is a lethal immunopathological disease caused by feline coronaviruses (FCoVs). Here, we describe a reverse genetics approach to study FIP by assessing the pathogenicity of recombinant type I and type II and chimeric type I/type II FCoVs. All recombinant FCoVs established productive infection in cats, and recombinant type II FCoV (strain 79-1146) induced FIP. Virus sequence analyses from FIP-diseased cats revealed that the 3c gene stop codon of strain 79-1146 has changed to restore a full-length open reading frame (ORF).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cats
  • Coronavirus, Feline / genetics*
  • Coronavirus, Feline / pathogenicity
  • Coronavirus, Feline / physiology
  • Feline Infectious Peritonitis / virology*
  • Reverse Genetics / methods*
  • Virulence