Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts

Exp Dermatol. 2012 May;21(5):331-6. doi: 10.1111/j.1600-0625.2012.01454.x.

Abstract

Cutaneous wound repair is a highly ordered and well-coordinated process involving various cell lineages and many molecular effectors. Cell-matrix interactions through integrin molecules provide key signals important for wound repair. Periostin is a matricellular protein that may provide signals important during tissue development and remodelling by interacting with several integrin molecules, via the phosphatidylinositol 3-kinase/Akt and MAP kinase pathways. In this study, we examined the role of periostin in the process of cutaneous wound repair using periostin-deficient mice and by analysing the effects of periostin on dermal fibroblasts. We first determined the expression profile and localization of periostin in a well-characterized wound repair model mice. Periostin was robustly deposited in the granulation tissues beneath the extended epidermal wound edges and at the dermal-epidermal junctions in wounded mice. Moreover, periostin-deficient mice exhibited delayed in vivo wound repair, which could be improved by direct administration of exogenous periostin. In vitro analyses revealed that loss of periostin impaired proliferation and migration of dermal fibroblasts, but exogenous supplementation or enforced periostin expression enhanced their proliferation. Combined, these results demonstrate that periostin accelerates the process of cutaneous wound repair by activating fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion Molecules / deficiency
  • Cell Adhesion Molecules / pharmacology
  • Cell Adhesion Molecules / physiology*
  • Cell Movement* / drug effects
  • Cell Proliferation* / drug effects
  • Cells, Cultured
  • Dermis / injuries*
  • Dermis / pathology
  • Fibroblasts / pathology*
  • In Vitro Techniques
  • Integrins / physiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitogen-Activated Protein Kinase Kinases / physiology
  • Models, Animal
  • Signal Transduction / physiology
  • Up-Regulation / physiology
  • Wound Healing / physiology*

Substances

  • Cell Adhesion Molecules
  • Integrins
  • Postn protein, mouse
  • Mitogen-Activated Protein Kinase Kinases