Comparison among three polymerase chain reaction assays on detection of DNA from Leishmania in biological samples from patients with American cutaneous leishmaniasis

Rev Soc Bras Med Trop. 2012 Mar-Apr;45(2):257-9. doi: 10.1590/s0037-86822012000200023.

Abstract

Introduction: The study analyzed positivity of polymerase chain reaction (PCR) on detection of DNA from Leishmania in patients' samples.

Methods: Extracted DNA was submitted to L150/L152, 13Y/13Z, and seminested PCR (snPCR).

Results: Results were evidenced by bands of approximately 120, 720, and 670 bp for L150/L152, 13Y/13Z, and snPCR, respectively. L150/L152, 13Y/13Z, and snPCR positivity indexes were 76.9, 56.4, and 9.2 (p>0.05), respectively, for suspected and 93.7, 68.7, and 84.4 (p<0.05), respectively, for confirmed.

Conclusions: Preliminary results showed that these assays, mainly L150/L152 and snPCR, can detect Leishmania DNA and carry potential on laboratory diagnosis of leishmaniasis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Protozoan / analysis*
  • Humans
  • Leishmania / genetics*
  • Leishmania / isolation & purification
  • Leishmaniasis, Cutaneous / diagnosis*
  • Polymerase Chain Reaction / methods*

Substances

  • DNA, Protozoan