Actively Q-switched self-frequency-doubling laser at 800 nm was first reported in an Er:Yb:YAl3(BO3)4 crystal by using an acousto-optical modulator. At incident pump power of 16 W and pulse repetition frequency of 1 kHz, 1600 nm fundamental pulse laser with energy of 130 μJ and width of 170 ns, and self-frequency-doubling 800 nm pulse laser with energy of 20 μJ and width of 96 ns were respectively achieved in a hemispherical resonator end-pumped by a 970 nm laser diode. Pulse characteristics of fundamental and self-frequency-doubling lasers at different pulse repetition frequencies were also investigated.