Abstract
A semimechanized simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonates as intermediates was developed and applied to the preparation of hybridization probes. The synthesis could be done with equivalent results (about 98% average yield per cycle) using regenerated H-phosphonates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Chromatography, High Pressure Liquid
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Genetic Techniques*
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Oligonucleotide Probes / chemical synthesis
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Oligonucleotides / chemical synthesis*
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Organophosphonates / chemistry
Substances
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Oligonucleotide Probes
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Oligonucleotides
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Organophosphonates