Antigenotoxic properties of two newly synthesized β-aminoketones against N-methyl-N'-nitro-N-nitrosoguanidine and 9-aminoacridine-induced mutagenesis

J Biochem Mol Toxicol. 2012 Jul;26(7):258-63. doi: 10.1002/jbt.21414. Epub 2012 May 29.

Abstract

The aim of this study was to determine the antigenotoxic potential of two newly synthesized β-aminoketones against N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 9-aminoacridine (9-AA)-induced mutagenesis. The mutant bacterial tester strains were MNNG-sensitive Escherichia coli WP2 uvrA and 9-AA-sensitive Salmonella typhimurium TA1537. Both test compounds showed significant antimutagenic activity at various tested concentrations. The inhibition rates ranged from 29.5% (compound 1: 2 mM/plate) to 47.5% (compound 2: 1.5 mM/plate) for MNNG and from 25.0% (compound 2: 1 mM/plate) to 52.1% (compound 2: 2.5 mM/plate) for 9-AA genotoxicity. Moreover, the mutagenicity of the test compounds was investigated by using the same strains. Neither test compound has mutagenic properties on the bacterial strains at the tested concentrations. Thus, the findings of the present study give valuable information about chemical prevention from MNNG and 9-AA genotoxicity by using synthetic β-aminoketones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminacrine / toxicity*
  • Antimutagenic Agents* / chemical synthesis
  • Antimutagenic Agents* / pharmacology
  • Cyclohexanones / chemical synthesis
  • Cyclohexanones / chemistry
  • Cyclohexanones / pharmacology*
  • Drug Antagonism
  • Escherichia coli / genetics
  • Methylnitronitrosoguanidine / toxicity*
  • Microbial Viability / drug effects*
  • Mutagenesis* / drug effects
  • Mutation / drug effects
  • Salmonella typhimurium / genetics

Substances

  • 2-((4-bromophenyl)((4-methylphenyl)amino)methyl)cyclohexanone
  • 2-((4-chlorophenyl)((4-methylphenyl)amino)methyl)cyclohexanone
  • Antimutagenic Agents
  • Cyclohexanones
  • Methylnitronitrosoguanidine
  • Aminacrine