Alternatively activated dendritic cells regulate CD4+ T-cell polarization in vitro and in vivo

Proc Natl Acad Sci U S A. 2012 Jun 19;109(25):9977-82. doi: 10.1073/pnas.1121231109. Epub 2012 Jun 1.

Abstract

Interleukin-4 is a cytokine widely known for its role in CD4(+) T cell polarization and its ability to alternatively activate macrophage populations. In contrast, the impact of IL-4 on the activation and function of dendritic cells (DCs) is poorly understood. We report here that DCs respond to IL-4 both in vitro and in vivo by expression of multiple alternative activation markers with a different expression pattern to that of macrophages. We further demonstrate a central role for DC IL-4Rα expression in the optimal induction of IFNγ responses in vivo in both Th1 and Th2 settings, through a feedback loop in which IL-4 promotes DC secretion of IL-12. Finally, we reveal a central role for RELMα during T-cell priming, establishing that its expression by DCs is critical for optimal IL-10 and IL-13 promotion in vitro and in vivo. Together, these data highlight the significant impact that IL-4 and RELMα can have on DC activation and function in the context of either bacterial or helminth pathogens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Polarity*
  • Cytokines / immunology
  • Dendritic Cells / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Humans
  • In Vitro Techniques
  • Macrophage Activation
  • Mice
  • Mice, Inbred C57BL
  • Signal Transduction

Substances

  • Cytokines