A technique of pancreas transplantation in the rat securing pancreatic juice for monitoring

Transpl Int. 1990 Oct;3(3):181-2. doi: 10.1007/BF00355468.

Abstract

In order to study exocrine pancreas graft function and cytological findings, a technique of vascularized pancreas transplantation with special reference to a pancreatic juice collecting system has been developed in the rat model. For this purpose, a catheter is introduced into the common bile duct, which is ligated close to the duodenum, thus covering all pancreatic ducts. This catheter is connected to a reservoir implanted subcutaneously, from which pancreatic juice can easily be aspirated. The amount of 0.7-1.2 cc of juice produced over a 24-h period has proven to be sufficient for various analyses and cytological examination.

MeSH terms

  • Animals
  • Monitoring, Physiologic / methods
  • Pancreas Transplantation / methods*
  • Pancreatic Juice / metabolism*
  • Rats