Molecular characterization and panton-valentine leucocidin typing of community-acquired methicillin-sensitive Staphylococcus aureus clinical isolates

J Clin Microbiol. 2012 Sep;50(9):3069-72. doi: 10.1128/JCM.00602-12. Epub 2012 Jun 20.

Abstract

Limited comprehensive molecular typing data exist currently for Panton-Valentine leucocidin (PVL)-positive, methicillin-sensitive Staphylococcus aureus (PVL-MSSA) clinical isolates. Characterization of PVL-MSSA isolates by multilocus sequence typing (MLST) and spa typing in this study showed a genetic similarity to PVL-positive, methicillin-resistant S. aureus (PVL-MRSA) strains, although three novel spa types and a novel MLST (ST1518) were detected. Furthermore, the detection of PVL phages and haplotypes in PVL-MSSA identical to those previously found in PVL-MRSA isolates highlights the role these strains may play as precursors of emerging lineages of clinical significance.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Bacterial Toxins / genetics*
  • Child
  • Child, Preschool
  • Community-Acquired Infections / microbiology*
  • Exotoxins / genetics*
  • Female
  • Haplotypes
  • Humans
  • Infant
  • Infant, Newborn
  • Leukocidins / genetics*
  • Male
  • Methicillin / pharmacology
  • Microbial Sensitivity Tests
  • Middle Aged
  • Molecular Epidemiology
  • Molecular Typing*
  • Prophages / genetics
  • Staphylococcal Infections / microbiology*
  • Staphylococcus Phages / genetics
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification
  • Virulence Factors / genetics*
  • Young Adult

Substances

  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Virulence Factors
  • Methicillin