Chromosome-specific subsets of human alphoid DNA identified by a chromosome 2-derived clone

M Rocchi; A Baldini; N Archidiacono; S Lainwala; O J Miller; D A Miller

doi:10.1016/0888-7543(90)90258-v

Chromosome-specific subsets of human alphoid DNA identified by a chromosome 2-derived clone

Genomics. 1990 Dec;8(4):705-9. doi: 10.1016/0888-7543(90)90258-v.

Authors

M Rocchi¹, A Baldini, N Archidiacono, S Lainwala, O J Miller, D A Miller

Affiliation

¹ Center for Molecular Biology, Wayne State University, Detroit, Michigan 48201.

PMID: 2276742
DOI: 10.1016/0888-7543(90)90258-v

Abstract

We have cloned an alphoid DNA fragment, pBS4D, from the DNA of a human-hamster hybrid cell line containing chromosome 2 as its only cytologically detectable human component. Under high stringency conditions, pBS4D hybridized in situ mostly to chromosome 2 and to a lesser extent to chromosomes 18 and 20. Restriction analysis using the DNA from selected somatic hybrid cell lines revealed that the genomic organization of this alphoid DNA differs on each of these three chromosomes.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Base Sequence
Blotting, Southern
Cell Line
Chromosomes, Human, Pair 2*
Cloning, Molecular
Cricetinae
DNA Probes
DNA, Satellite / genetics*
Humans
Hybrid Cells
Molecular Sequence Data

Chromosome-specific subsets of human alphoid DNA identified by a chromosome 2-derived clone

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Abstract

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