Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro

Trans R Soc Trop Med Hyg. 2012 Oct;106(10):632-5. doi: 10.1016/j.trstmh.2012.05.004. Epub 2012 Jul 11.

Abstract

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.

MeSH terms

  • Antigens, Bacterial / metabolism*
  • Bacterial Proteins / metabolism*
  • Cells, Cultured
  • Fluorescent Antibody Technique, Indirect*
  • Humans
  • Orientia tsutsugamushi / isolation & purification*
  • Orientia tsutsugamushi / metabolism
  • Reproducibility of Results
  • Scrub Typhus / diagnosis*
  • Scrub Typhus / metabolism
  • Sensitivity and Specificity

Substances

  • Antigens, Bacterial
  • Bacterial Proteins