Prospective study of thrombospondin-1 mimetic peptides, ABT-510 and ABT-898, in dogs with soft tissue sarcoma

J Vet Intern Med. 2012 Sep-Oct;26(5):1169-76. doi: 10.1111/j.1939-1676.2012.00966.x. Epub 2012 Jul 21.

Abstract

Background: Exposure to anti-angiogenic thrombospondin-1 (TSP-1) mimetic peptides (MPs) has resulted in sporadic anti-tumor activity in humans and dogs.

Hypothesis: Novel TSP-1 MPs formulations will be safe, tolerated, and clinically active in soft tissue sarcoma (STS) in dogs.

Animals: Sixty-two client-owned dogs with measurable STS were enrolled, excluding hemangiosarcoma.

Methods: A prospective, single agent, multicenter, open-label study assessing ABT-510 bolus, ABT-898 bolus, or ABT-898 depot formulations of TSP-1 in dogs. Endpoints included tolerability, antitumor activity, and the assessment of ability of clinical covariates and circulating endothelial cells (CEC) concentration to predict tumor response.

Results: Two non-dose-limiting toxicoses possibly attributed to treatment were observed (keratitis and osteoarthritis). Antitumor activity (10/44 = 23% responses) was observed in study subjects who received treatment for >28 days (n = 44) including both partial (7) and minimal responses (3). Responses were disproportionately seen in dogs receiving ABT-898 formulations (9/28 = 32%) versus those receiving ABT-510 (1/16 = 6%; P < .045). Disease stabilization for >84 days was also documented (8/44 = 18%). Slow rates of tumor progression before study entry correlated with anti-tumor activity in treated dogs, whereas no significant association was found between changes in total CEC concentration and tumor response (P = .28) or time to progression (P = .42).

Conclusions and clinical importance: Safely achieved antitumor activity was documented with TSP-1 MPs in dogs with STS. The most notable activity was achieved with the ABT-898 formulations.

Publication types

  • Clinical Trial
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiogenesis Inhibitors / administration & dosage
  • Animals
  • Antineoplastic Agents / administration & dosage
  • Biomimetic Materials / administration & dosage
  • Blood Cell Count / veterinary
  • Cohort Studies
  • Dog Diseases / drug therapy*
  • Dog Diseases / metabolism
  • Dog Diseases / pathology
  • Dogs
  • Female
  • Male
  • Oligopeptides / administration & dosage*
  • Prospective Studies
  • Sarcoma / drug therapy
  • Sarcoma / metabolism
  • Sarcoma / pathology
  • Sarcoma / veterinary*
  • Soft Tissue Neoplasms / drug therapy
  • Soft Tissue Neoplasms / metabolism
  • Soft Tissue Neoplasms / pathology
  • Soft Tissue Neoplasms / veterinary*
  • Thrombospondin 1 / metabolism*

Substances

  • Angiogenesis Inhibitors
  • Antineoplastic Agents
  • NAc-Sar-Gly-Val-(d-allo-Ile)-Thr-Nva-Ile-Arg-ProNEt
  • Oligopeptides
  • Thrombospondin 1
  • acetyl-glycyl-valyl-allo-isoleucyl-seryl-glutaminyl-isoleucyl-arginyl-prolyl-cysteinamide