A mass spectrometry-based immunosensor for bacteria using antibody-conjugated gold nanoparticles

Chem Commun (Camb). 2012 Sep 7;48(69):8712-4. doi: 10.1039/c2cc34120d. Epub 2012 Jul 24.

Abstract

We have developed a simple mass spectrometry-based immunosensor using antibody (Ab)-modified gold nanoparticles (Ab-Au NPs) for the rapid quantitation of bacteria via the analysis of Au clusters under pulsed laser irradiation.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Immobilized / chemistry
  • Antibodies, Immobilized / immunology*
  • Bacteria / immunology
  • Bacteria / isolation & purification*
  • Bacterial Infections / diagnosis
  • Food Microbiology
  • Gold / chemistry*
  • Humans
  • Immunoassay / methods*
  • Nanoparticles / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Urine / microbiology
  • Water Microbiology

Substances

  • Antibodies, Immobilized
  • Gold