[Comparative study between primary mediastinal B-cell lymphoma and non-mediastinal diffuse large B-cell lymphoma by immunoglobulin gene rearrangement and Epstein-Barr virus infection detection]

Zhonghua Bing Li Xue Za Zhi. 2012 Jun;41(6):361-5. doi: 10.3760/cma.j.issn.0529-5807.2012.06.001.
[Article in Chinese]

Abstract

Objective: To investigate the differences between primary mediastinal B-cell lymphoma (PMBCL) and non-mediastinal conventional diffuse large B-cell common lymphoma (DLBCL) in immunoglobulin gene rearrangement and EB virus infections.

Methods: Twenty cases of PMBCL and 30 cases of non-mediastinal DLBCL were collected from September, 2000 to May, 2011. Pathological data were retrospectively analysed. Immunoglobulin heavy chain and light chain gene rearrangements and EBER in-situ hybridization were performed.

Results: Six of 20 cases of PMBCL showed monoclonal gene rearrangement, all of which were weakly detected. Twenty-seven of 30 cases of ordinary diffuse large B-cell lymphoma showed monoclonal gene rearrangement, which were strongly detected (90.0%). Only 1 of 20 cases PMBCL and 2 of 30 cases of DLBCL were positive for EBER in-situ hybridization.

Conclusions: The detection rate of immunoglobulin gene rearrangement is significantly lower in PMBCL than that of non-mediastinal DLBCL. However, EB virus infection rates are very low in both types of lymphomas.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Adult
  • Epstein-Barr Virus Infections*
  • Female
  • Gene Rearrangement, B-Lymphocyte*
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • In Situ Hybridization
  • Lymphoma, B-Cell* / genetics
  • Lymphoma, B-Cell* / virology
  • Lymphoma, Large B-Cell, Diffuse* / classification
  • Lymphoma, Large B-Cell, Diffuse* / genetics
  • Lymphoma, Large B-Cell, Diffuse* / virology
  • Male
  • Mediastinal Neoplasms* / genetics
  • Mediastinal Neoplasms* / virology
  • Middle Aged
  • RNA, Viral / analysis
  • Retrospective Studies
  • Young Adult

Substances

  • RNA, Viral