Comparison of immunochromatography, PCR and culture methods for the detection of Campylobacter bacteria

Shuvra Kanti Dey; Shuichi Nishimura; Shoko Okitsu; Satoshi Hayakawa; Masashi Mizuguchi; Hiroshi Ushijima

doi:10.1016/j.mimet.2012.09.034

Comparison of immunochromatography, PCR and culture methods for the detection of Campylobacter bacteria

J Microbiol Methods. 2012 Dec;91(3):566-8. doi: 10.1016/j.mimet.2012.09.034. Epub 2012 Oct 8.

Authors

Shuvra Kanti Dey¹, Shuichi Nishimura, Shoko Okitsu, Satoshi Hayakawa, Masashi Mizuguchi, Hiroshi Ushijima

Affiliation

¹ Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.

PMID: 23059063
DOI: 10.1016/j.mimet.2012.09.034

Abstract

A total of 463 fecal specimens from human patients were tested for the presence of Camphylobacter by a commercial immunochromatography kit (ImmunoCard STAT! CAMPY), polymerase chain reaction (PCR) and conventional bacteriological (CB) methods. Using culture as the standard of reference, the ImmunoCard STAT! CAMPY assay had a sensitivity of 86% and a specificity of 100%. On the other hand, using PCR as the standard of reference, ImmunoCard STAT! CAMPY assay had a sensitivity of 90.5% and a specificity of 100%.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Campylobacter / genetics
Campylobacter / growth & development
Campylobacter / isolation & purification*
Campylobacter Infections / diagnosis
Campylobacter Infections / microbiology*
Chromatography, Affinity / methods*
Colony Count, Microbial / methods*
Feces / microbiology*
Humans
Polymerase Chain Reaction / methods*