Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp

Mol Cell Probes. 2013 Feb;27(1):68-70. doi: 10.1016/j.mcp.2012.10.001. Epub 2012 Oct 9.

Abstract

A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L. monocytogenes.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques / methods*
  • DNA, Bacterial / analysis
  • Food Microbiology
  • Listeria monocytogenes / classification*
  • Listeria monocytogenes / genetics*
  • Listeria monocytogenes / isolation & purification
  • Real-Time Polymerase Chain Reaction / methods*
  • Serotyping / methods
  • Species Specificity

Substances

  • Bacterial Proteins
  • DNA, Bacterial