Determination of GABA(Aα1) and GABA (B1) receptor subunits expression in tissues of gilts during the late gestation

Mol Biol Rep. 2013 Feb;40(2):1377-84. doi: 10.1007/s11033-012-2181-z. Epub 2012 Oct 21.

Abstract

GABA(Aα1) and GABA(B1) receptor subunits are responsible for most behavioral, physiological and pharmacological effects of GABA receptors. We investigated the expression of GABA(Aα1) and GABA(B1) receptor subunits in different tissues of gilts during late pregnancy in hot summer. The mRNA abundance of GABA(Aα1) receptor subunit in different tissues of gilts at d 90 and d 110 of gestation was as follows: d 90: brain > lung > liver > ovary > spleen > kidney > heart; d 110: brain > lung > spleen > liver > ovary > kidney > heart. And, the mRNA abundance of GABA(B1) receptor subunit was as follows: d 90: spleen > lung > brain > kidney > ovary > liver > heart; d 110: spleen > lung > kidney > brain > ovary > liver > heart. The results in this trial indicated that the GABA(Aα1) receptor subunit was abundantly expressed in brain, while GABA(B1) receptor subunit was abundant in spleen and lung of gilts during late gestation. There were no gestation stage-dependent effects on GABA(Aα1) and GABA(B1) receptor subunits expression in all tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Female
  • Gene Expression Regulation
  • Gene Expression*
  • Gestational Age
  • Lung / metabolism
  • Organ Specificity
  • Pregnancy / metabolism*
  • Protein Subunits
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, GABA-A / genetics*
  • Receptors, GABA-A / metabolism
  • Receptors, GABA-B / genetics*
  • Receptors, GABA-B / metabolism
  • Spleen / metabolism
  • Sus scrofa / metabolism*

Substances

  • Protein Subunits
  • RNA, Messenger
  • Receptors, GABA-A
  • Receptors, GABA-B